目的 采用离子迁移谱(ion mobility spectrometry,IMS)技术,建立中药材中金胺O、苋菜红、日落黄非法染色的快速检测方法 。方法 采用离子迁移谱技术,设定条件为负离子下离子源电压1 800 V、正离子下离子源电压2 300 V、漂移管电压7 500 V、进气温度 180 ℃、漂移管温度180 ℃、栅电压45 V、狭缝脉冲宽度120 μs、漂移管流速1.2 L·min^-1、排气泵0.8 L·min^-1、运行时间30 s、光谱范围25 ms,样品经甲醇提取,直接进样,与对照品的迁移时间进行比对分析,判断是否有色素的非法染色。结果 可以实现3种色素单一存在或同时存在染色时的检测鉴定,同时根据信噪比(S/N)=3确定出3种非法染色色素低检出限。结论 建立的离子迁移谱检测方法 快速、简便、稳定,可以用于中药材中非法染色色素金胺O、日落黄和苋菜红的初步筛查。

OBJECTIVE To develop a fast detecting method for three dyes (auramine O,amaranth and sunset yellow) illegally added into Chinese herbal medicine by ion mobility spectrometry (IMS). METHODS The analysis was performed on ion mobility spectrometry,with source voltage 1 800 V for negative source and 2 300 V for positive source,drift tube voltage 7 500 V,gas inlet temperature 180 ℃,drift tube temperature 180 ℃,gate voltage 45 V,gate pulse width 120 μs,drift gas flow 1.2 L·min^-1,exhaust pump 0.8 L·min^-1,run time 30 s and spectrum length 25 ms. The samples were extracted by methanol,and then injected into the IMS system. The judgement of whether dye was added or not was made by comparing the migration time of the test samples with that of the reference substances. RESULTS The auramine O,amaranth and sunset yellow could be rapidly identified. The minimum detection concentration of each compound was determined according to the signal to noise ratio (S/N) of 3. CONCLUSION The IMS method for detecting auramine O,amaranth and sunset yellow illegally added into Chinese herbal medicine is simple,rapid and expected to be used as an initial screening method in drug rapid detecting system.